Xylanase Production by Solid-State Fermentation for the Extraction of Xylooligosaccharides from Soybean Hulls

Research background. The development of a novel process for the production xylooligosaccharides based on 4R concept is made possible by integration numerous techniques, especially enzymatic modification, together with physical pretreatment renewable materials. This study aims to integrate use agricultural wastes xylanase new strain Penicillium sp. and value-added products, xylooligosaccharides. Experimental approach. To produce xylanase, solid-state fermentation was performed using wheat bran as substrate. In order obtain most active crude extract, time frame cultivation first adjusted. Then, downstream purification developed combining different membrane separation units size exclusion chromatography. Further characterization included determination optimal pH temperature, molecular mass purified analysis kinetic parameters. Subsequently, hydrolytic ability partially in hydrolysis alkali-extracted hemicellulose from soybean hulls investigated. Results conclusions. Our results show that rubens produced extracellular at yield 21 U/g during fermentation. Using two ultrafiltration membranes 10 3 kDa combination chromatography, 49 % 13-fold achieved. (35 kDa) cleaved linear bonds β-(1→4) beechwood xylan maximum rate 0.64 μmol/(min·mg) Michaelis constant 44 mg/mL. At pH=6 45 °C, showed its activity. high hydrolyze fraction isolated hulls, confirmed thin-layer hydrothermally pretreated hydrolysate, content XOS degrees polymerization detected, while non-pretreated xylotriose glucose confirmed. Novelty scientific contribution. Future research focused creating pathways processes convert materials into products can draw our findings.